PMC

Figure 1Weights of two example units on a neural manifold of position and velocity (parameters: ).

Fig. 7

Figure 3Binge alcohol and acidemia exposure effects on Fetal Femoral and Tibial Max Breaking Force per Unit Fetal Body Weight (N/kg)

Fig. 3(A) Abstract diagram of the three-input integration gate motif and its DNA implementation. The black numbers indicate the initial relative concentration. (B) Illustration of the integration gate. The inputs signal consisted of three parts: X1, X2 and X3; the weights consisted of three parts: W1, W2 and W3. All substrate strands (S1a,b, S2d,b, S3e,b) were labelled with fluorophore FAM at 5′-end and quencher BHQ1 at 3′-end for fluorescent signal determination; L* was the normalized output. (C) Native PAGE analysis of the integration gate. The strands and complex involved were labelled above the lane number. Lane 1, weight unit mixture (W1 + W2 + W3); lane 2, X1 was added to the weight unit mixture; lane 3, X2 was added to the weight unit mixture; lane 4, X3 was added to the weight unit mixture; lane 5, X1 and X2 were added to the weight unit mixture; lane 6, X1 and X3 were added to the weight unit mixture; lane 7, X2 and X3 were added to the weight unit mixture; lane 8, all signals were added to the weight unit mixture; lane 9, normalized product strand L. (D) Normalized fluorescence of the integration gate. The standard concentration was 1× = 0.2 μM, [W1] : [W2] : [W3] : [X1] : [X2] : [X3] = 1 : 1 : 1 : 1 : 1 : 1. The sum value was inferred from the fluorescent signal. Curve (1) had no input signals added; Curves (2)–(4) had only one input signal added; Curves (5)–(7) had two kinds of input signals added; Curve (8) had all input signals added.